Hepatoprotective Activity of Bergenia ciliata Roots in Ethanol Induced Oxidative Stress and Hepatotoxicity in Rats

Authors

  • Pravesh Manger Department of Pharmacology, Karnataka College of Pharmacy, Bangalore, Karnataka, India
  • Deepak Kumar Jha Department of Pharmacology, Karnataka College of Pharmacy, Bangalore, Karnataka, India

DOI:

https://doi.org/10.25004/IJPSDR.2024.160304

Keywords:

Bergenia ciliata, Hepatoprotective, Ethanol, Antioxidant, Phytochemistry

Abstract

Liver disease (LD) is one of the main causes of mortality and modality in worldwide. Around 500 million people worldwide are thought to have chronic hepatitis infections, which cause over a million deaths a year.  Treatments for LD must be developed with a new approach to cure or prevent the progression of the disease without any consequences. Currently, this study's purpose is to examine and confirm the methanolic extract of Bergenia Ciliata root’s ability to protect rats from ethanol induced hepatotoxicity. Wistar rats were administered 1 cc of 30% ethyl alcohol in all the groups except the normal control P.O. once a day for 40 days in order to cause hepatoxicity. After confirmation of LD, the methanolic extract of Bergenia ciliata (MEBC) roots and the standard drug silymarin (0.1 g/kg, b.w., orally) were given twice daily for 21 days. Liver weight, body weight, Serum liver enzymes like SGPT, SGOT, serum ALP, total protein, albumin, and antioxidant enzymes were assayed to investigate the hepatoprotective effect, followed by liver histopathology to evaluate the hepatic architecture and all alignments and inflammatory cells. The protective markers of all the rats treated with alcohol showed a substantial increase, and the rats administered MEBC showed a remarkable recovery towards an almost normal level. These findings suggest that MEBC protected the structural integrity of the hepatocellular membrane and ethanol-damaged liver cells cellular architecture, which was supported by histological analysis. The present study demonstrates that the methanolic extract Bergenia Ciliata roots possess hepatoprotective property.

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References

Ali M, Khan T, Fatima K. Selected hepatoprotective herbal medicines: Evidence from ethnomedicinal applications, animal models, and possible mechanism of actions. Wiley. 2018;32(2):199-215. Available from: doi.org/ 10.1002/ptr.5957

Singh R, Rao HS. Hepatoprotective effect of the pulp/seed of Aegle Marmelos correa ex Roxb against carbon tetrachloride induced liver damage in rat. International Journal of Green Pharmacy. 2008;2(4);232-234. Available from: doi.org/10.22377/ijgp.v2i4.15

Osna NA, Donohue TM, and Kharbanda KK. Alcoholic Liver Disease: Pathogenesis and Current Management. Alcohol Res. 2017;38(2):147-161. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5513682

Latief U, Tung GK, Singh H, Sharief Per T and Subheet Kumar Jain, Bergenia ciliata as a future candidate for liver diseases: The Journal of Basic and Applied Zoology 2022;83(17):1-14. Available from: doi.org/10.1186/s41936-022-00282-x.

Izzo AA, Hoon‐Kim S, Radhakrishnan R, Williamson EM. A critical approach to evaluating clinical efficacy, adverse events and drug interactions of herbal remedies. Phytotherapy research. 2016;30(5):691-700. Available from: doi.org/ 10.1002/ptr.5591.

Helmstädter A, Staiger C. Traditional use of medicinal agents: a valid source of evidence. Drug Discovery Today 2014;19(1):4-7. Available from: doi.org/ 10.1016/j.drudis.2013.07.016.

Koul B, Kumar A, Yadav D, Jin JO. Bergenia genus: Traditional uses, phytochemistry and pharmacology. Molecules. 2020;25(23):5555. Available from: doi.org/ 10.3390/molecules25235555.

Petta S, Muratore C, Craxi A. Non-alcoholic fatty liver disease pathogenesis: the present and the future. Dig Liver Dis. 2009;41(9):615-25. Available from: doi.org/10.1016/j.dld.2009.01.004.

Mirzaagha F, Azali SH, Islami F, Zamani F, Khalilipour E, Khatibian M, Malekzadeh R. Coeliac disease in autoimmune liver disease: a cross-sectional study and a systematic review. Digestive and Liver Disease. 2010;42(9):620-3. Available from: doi.org/ 10.1016/j.dld.2010.02.006.

Fong CY, Gauthaman K, Bongso A. Teratomas from pluripotent stem cells: a clinical hurdle. Journal of cellular biochemistry. 2010;111(4):769-81. Available from: doi.org/ 10.1002/jcb.22775.

Negi VS, Elluru S, Siberil S, Graff Dubois S. Intravenous immunoglobulin: an update on the clinical use and mechanisms of action. Journal of clinical immunology. 2007;27:233-45. Available from: doi.org/ 10.1007/s10875-007-9088-9.

Phull AR, Abbas Q, Ali A, Raza H, Zia M, & Haq IU. Antioxidant, cytotoxic and antimicrobial activities of green synthesized silver nanoparticles from crude extract of Bergenia ciliata. Future Journal of Pharmaceutical Sciences 2016;2(1):31–36. Available from: doi.org/10.1016/j.fjps.2016.03.001

Hussain A, Kanth M., Shrivastva PK, Sharma M, Tripath J, & Khan MA. Phytochemical analysis of the rhizomes of Bergenia ciliata (How) Sternb. Journal of Drug Delivery and Therapeutics 2019;9(3):412–6. Available from: doi.org/10.22270/jddt.v9i3.2687

Rajkumar V, Guha G, & Kumar RA. Anti-neoplastic activities of Bergenia ciliata rhizome. Journal of Pharmacy Research 2011;4(2):443–5. Available from: https://www.researchgate.net/publication/215550807.

Dharmender R, Madhavi T, Reena A, & Sheetal A. Simultaneous quantification of Bergenin, (+)-Catechin, Gallicin and Gallic acid; and quantification of β-Sitosterol using HPTLC from Bergenia ciliate. Pharmaceutica Analytica Acta 2010;1:1-9. Available from: doi.org/10.4172/2153-2435.1000104

Thurman RG, Bradford BU et.al. Mechanisms of Alcohol-Induced Hepatotoxicity: Studies in Rats. Frontiers in Bioscience: 1999;4(1):42. Available from: doi.org/ 10.2741/A478.

Bagul MS, Ravishankara MN, Padh H, Rajani M. Phytochemical evaluation and free radical scavenging properties of rhizome of Bergenia ciliate. Journal of Natural Remedies 2003;3(1):83 – 89.

Available from:doi.org/10.18311/jnr/2003/369

Gupta HS, Rajashekha U, Jha DK. Assessing the Effect of Portulaca oleracea Seed Extract for Alleviating Collagen Type-II Induced Arthritis in Female Wistar Rat. IJPSDR 2024;16(2):163-170.

Available from: doi.org/10.25004/IJPSDR.2024.160205.

Ali AAM, Rajashekhar U, Jha DK. An Investigation of Type II Anti-diabetic Activity of Biologically Active Phytochemical(s) of Coccinia indica Fruits in High Sugar Diet-Induced Diabetic Rats. IJPSDR 2024; 16(1): 52-58. Available from: doi.org/10.25004/IJPSDR.2024.160108.

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Published

30-05-2024

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Section

Research Article

How to Cite

“Hepatoprotective Activity of Bergenia Ciliata Roots in Ethanol Induced Oxidative Stress and Hepatotoxicity in Rats”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 16, no. 3, May 2024, pp. 335-40, https://doi.org/10.25004/IJPSDR.2024.160304.