Effect of Ethanolic Extract of Alternanthera sessilis on Behavioral Parameters in Reserpine-Induced Stroke and Seizure Rat Model

Authors

  • Syed LAS Department of Physiology, Vinayaka Mission's Medical College and Hospitals, Vinayaka Mission's Research Foundation (Deemed to be University) Karikal-609609, Tamil Nadu, India
  • Sheela JPP Department of Physiology, Vinayaka Mission Kirupananda Variyar Medical College, Salem-636308, Tamil Nadu, India
  • Balaji PV Department of Physiology, Vinayaka Mission's Medical College and Hospitals, Vinayaka Mission's Research Foundation (Deemed to be University) Karikal-609609, Tamil Nadu, India
  • Aravind KR Department of Physiology, Rajamuthaiya Medical College, Chidambaram-608002, Tamil Nadu, India
  • Thirumaran M Department of Physiology, Vinayaka Mission’s Medical College and Hospitals, Vinayaka Mission’s Research Foundation (Deemed to be University) Karikal, Tamil Nadu, India
  • Manibalan V Department of Physiology, Vinayaka Mission's Medical College and Hospitals, Vinayaka Mission's Research Foundation (Deemed to be University) Karikal-609609, Tamil Nadu, India

DOI:

https://doi.org/10.25004/IJPSDR.2024.160310

Keywords:

Seizures, Stroke, Reserpine, Alternanthera sessilis

Abstract

The primary aim of our study was to delve into the neuroprotective potential of an ethanolic extract sourced from Alternanthera sessilis, particularly in alleviating behavioural impairments induced by reserpine in rat models simulating stroke and seizures. Our investigation involved categorizing rats into five distinct groups, each consisting of six individuals, with separate sets allocated for different experimental interventions. Seizures and stroke-like symptoms were induced by administering reserpine, after which the rats underwent treatment with varying doses of Alternanthera sessilis ethanolic extract. We conducted a comprehensive assessment of various behavioural parameters, including antiepileptic activity, motor function, overall well-being, rota rod performance, closed field activity, and grip strength. Significantly, our results uncovered substantial improvements in antiepileptic activity, motor function, and overall health of the intervention group, which received the extract, to the control group after the administration period. Additionally, enhancements were noted in rota rod performance, closed field activity, and grip strength, indicative of a marked enhancement in neuroprotective effects. These encouraging findings highlight the potential therapeutic efficacy of Alternanthera sessilis extract in managing stroke and seizure disorders. Nevertheless, further extensive investigations are imperative to unravel the precise mechanisms underlying these observed enhancements. Furthermore, future research endeavors should focus on delineating the specific therapeutic applications of Alternanthera sessilis extract, thus facilitating its potential integration into clinical practice for the management of stroke and seizure-related conditions. This would pave the way for a more comprehensive understanding of its therapeutic benefits and broaden its scope for clinical utility.

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References

Ghulaxe Y, Joshi A, Chavada J, Huse S, Kalbande B, Sarda PP. Understanding focal seizures in adults: A comprehensive review. Cureus: Innovations in Neurology. 2023;15(11):e48173. DOI: 10.7759/cureus.48173.

Amengual-Gual M, Fernández IS, Loddenkemper T. Patterns of epileptic seizure occurrence. Brain Research. 2019;1703:3-12. https://doi.org/10.1016/j.brainres.2018.02.032.

Ahmadzade AM, Pourali G, Mirheidari SB, Shirazinia M, Hamedi M, Mehri A, Amirbeik H, Saghebdoust S, Tayarani-Najaran Z, Sathyapalan T, Forouzanfar F, Sahebkar A. Medicinal plants for the treatment of neuropathic pain: A review of randomized controlled trials. Curr Pharm Biotechnol. 2023 Jul 14. doi: 10.2174/1389201024666230714143538.

Mead GE, Sposato LA, Sampaio Silva G, Yperzeele L, Wu S, Kutlubaev M, Cheyne J, Wahab K, Urrutia VC, Sharma VK, Sylaja PN, Hill K, Steiner T, Liebeskind DS, Rabinstein AA. A systematic review and synthesis of global stroke guidelines on behalf of the World Stroke Organization. Int J Stroke. 2023 Jun;18(5):499-531. doi: 10.1177/17474930231156753.

Rost NS, Brodtmann A, Pase MP, van Veluw SJ, Biffi A, Duering M, et al. Post-stroke cognitive impairment and dementia. Circulation Research. 2022; 130(8):1252-1271. https://doi.org/10.1161/CIRCRESAHA.122.319951

Rajendran R, Abirami M, Santhini E, Dinesh Kirupha S, Vadodaria K. Evaluation of the bioactive properties of Alternanthera sessilis extract and development of sodium alginate–Alternanthera sessilis membrane for wound management. Journal of Polymer Engineering. 2020;40(6):507-518. https://doi.org/10.1515/polyeng-2019-0252

Hwong CS, Leong KH, Aziz AA, Junit SM, Noor SM, Kong KW. Alternanthera sessilis: Uncovering the nutritional and medicinal values of an edible weed. Journal of Ethnopharmacology. 2022. https://doi.org/10.1016/j.jep.2022.115608v 15; 298:115608.

Muhammad Atif Randhawa, Ammar Ahmad Khan, Muhammad Sameem Javed, Muhammad Wasim Sajid, Chapter 18 - Green Leafy Vegetables: A Health Promoting Source, Editor(s): Ronald Ross Watson, Handbook of Fertility, Academic Press,2015,205-220, https://doi.org/10.1016/B978-0-12-800872-0.00018-4.

Suroowan S, Pynee KB, Mahomoodally MF. A comprehensive review of ethnopharmacologically important medicinal plant species from Mauritius. South African Journal of Botany. 2019;122:189-213. https://doi.org/10.1016/j.sajb.2019.03.024.

Alrashdi J, Albasher G, Alanazi MM, Al-Qahtani WS, Alanezi AA, Alasmari F. Effects of Rosmarinus officinalis L. extract on neurobehavioral and neurobiological changes in male rats with pentylenetetrazol-induced epilepsy. Toxics. 2023;11(10):826. https://doi.org/10.3390/toxics11100826.

Ralta A, Prakash A, Kumar_M P, Kumar R, Sarma P, Bhatia A, Medhi B, Chakrabarti A. Neuroprotective Effect of Celastrus Paniculatus Seed Extract on Epilepsy and Epilepsy-associated Cognitive Deficits. Basic and Clinical Neuroscience. 2023;14(1):155. DOI: 10.32598/bcn.2021.3154.1.

Olurankinse AI, Imoru JO, Oyemitan IA. Behavioural, Sedative and Anticonvulsant Activities of Ethanol Extract of the Leaf of Lagenaria breviflora (Benth) in Mice. Nigerian Journal of Pharmaceutical Research. 2023; 19(1):79-92. https://dx.doi.org/10.4314/njpr.v19i1.8.

Kargar Jahromi H, Khorram A, Atashpoor S, Jalali M, Kargar Jahromi Z, Mohammadi F, Serpoosh MA, Sadeghi N. The Effect of Hydroalcoholic Extract of Salep on Pentylenetetrazole-Induced Seizure in Adult Male Rats. Iranian Journal of Neurosurgery. 2023; 9:1-9.DOI: ‎ 10.32598/irjns.9.1.

Salile SS, Lee HJ, Alberts PS, Abula T, Raimondo JV, Stafford GI. In vitro and in vivo anti-seizure activity of hydromethanolic extract and fractions of Pterolobium stellatum. Journal of Ethnopharmacology. 2023; 304:116073. DOI: 10.1016/j.jep.2022.116073.

Oubella K, Benrazzouk K, Bagri A, Chait A. Effect of Cuminum cyminum and Rhus pentaphylla extracts on status epilepticus induced by intrahippocampal injection of kainic acid in rat. Bangladesh Journal of Pharmacology. 2023;18(3):105-112. DOI: 10.3329/bjp.v18i3.67165.

Rashidi R, Mahdinezhad MR, Sobhanifar MA, Marefati N, Rakhshandeh H, Hosseini M. The Impact of Artemisia absinthium Hydro-ethanolic Extract on Oxidative Stress in the Brain in a Seizure Model Induced by Pentylenetetrazole in Mice. Journal of Chemical Health Risks. 2023;13(4):671-682.DOI: 10.22034/jchr.2022.1935870.1364.

Mansouri S, Hosseini M, Alipour F, Beheshti F, Rakhshandeh H, Mohammadipour A, Anaeigoudari A, Jalili-Nik M, Khazdair MR, Jahani A. Neuroprotective effects of the fractions of Ocimum basilicum in seizures induced by pentylenetetrazole in mice. Avicenna Journal of Phytomedicine. 2022;12(6):614. doi: 10.22038/AJP.2022.20470.

Alkhudhayri A, Abdel Moneim AE, Rizk S, Bauomy AA, Dkhil MA. The neuroprotective effect associated with Echinops spinosus in an acute seizure model induced by pentylenetetrazole. Neurochemical Research. 2023; 48(1):273-283. DOI: 10.1007/s11064-022-03738-2.

Patel C, Thakur K, Shagond L, Acharya S, Ranch K, Boddu SH. Effect of Shorea robusta resin extract in 3-nitropropionic acid-induced Huntington’s disease symptoms in Sprague-Dawley rats. Research in Pharmaceutical Sciences. 2023;18(3):303-316. doi: 10.4103/1735-5362.371586.

Nguyen LT, Le XT, Pham HN, Van Nguyen T, Nguyen PT, Van Thi Pham A, Nguyen TB, Matsumoto K. Therapeutic effects of a standardized-flavonoid Diospyros kaki L.f. leaf extract on transient focal cerebral ischemia-induced brain injury in mice. Journal of Natural Medicines. 2023. https://doi.org/10.1007/s11418-023-01699-6.

Nakyam T, Wattanathorn J, Thukham-mee W. Neuroprotective Effect of Polyherbal Recipe Containing Ginger, Chinese Date, and Wood Ear Mushroom against Ischemic Stroke with Metabolic Syndrome Condition via Epigenetic Modification of Inflammation and Oxidative Stress. BioMed Research International. 2022. https://doi.org/10.1155/2022/8940303.

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Published

30-05-2024

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Section

Research Article

How to Cite

“Effect of Ethanolic Extract of Alternanthera Sessilis on Behavioral Parameters in Reserpine-Induced Stroke and Seizure Rat Model”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 16, no. 3, May 2024, pp. 387-92, https://doi.org/10.25004/IJPSDR.2024.160310.